研究者業績

安増 茂樹

ヤスマス シゲキ  (Yasumasu Shigeki)

基本情報

所属
上智大学 理工学部物質生命理工学科 教授
学位
理学博士(上智大学)

連絡先
s-yasumahoffman.cc.sophia.ac.jp
研究者番号
00222357
J-GLOBAL ID
200901055266938036
researchmap会員ID
1000293741

授業科目は、理工学総論I、分子遺伝学、発生生物学等を担当している。授業は、プリントを配布しそれに沿って行う。単元の終了時にビデオまたはパワーポイントを用いる授業を加え、復習を兼ねて映像により理解を深めるよう努力している。同時に簡単な問題を出し回答させる。その際、学生よりの質問を受け付けることで学生の理解度を把握しつつ授業を展開する。

(研究テーマ)
孵化酵素の分子生物学
孵化酵素の卵膜分解機構


研究キーワード

 3

学歴

 1

受賞

 1

論文

 107
  • Shigeki Yasumasu, Miyuki Horie, Mayuko Horie, Kodai Sakuma, Chihiro Sato, Hikari Sato, Taiki Nakajima, Tatsuki Nagasawa, Mari Kawaguchi, Ichiro Iuchi
    The Journal of Biochemistry 2024年9月16日  
    Abstract During the fertilization of fish eggs, the hardening of the egg envelope is mediated by transglutaminase (hTGase). After fertilization, TGase undergoes processing. We isolated hTGase from extracts of unfertilized and water-activated rainbow trout eggs. Rainbow trout hTGase (Rt-hTGase) appeared as an 80 kDa protein, and its processed form was 55 kDa. Their N-terminal amino acid sequences were nearly identical, suggesting processing in the C-terminal region. The specific activities were not significantly different, indicating that C-terminal processing does not activate the enzyme itself. We cloned the cDNA by reverse transcription polymerase chain reaction (RT-PCR) using degenerate primers followed by RACE-PCR. The deduced amino acid sequence of the cDNA was similar to that of factor XIII subunit A (FXIIIA). Molecular phylogenetic and gene syntenic analyses clearly showed that hTGase was produced by duplication of FXIIIA during the evolution to Teleostei. The 55 kDa processed form of Rt-hTGase is predominantly composed of an enzyme domain predicted from the amino acid sequence of the cDNA. It is hypothesized that the C-terminal domain of Rt-hTGase binds to egg envelope proteins, and that processing allows the enzyme to move freely within the egg envelope, increasing substrate–enzyme interaction and thereby accelerating hardening.
  • Sakuto Yamanaka, Mari Kawaguchi, Shigeki Yasumasu, Kenji Sato, Masato Kinoshita
    Journal of Experimental Zoology Part B: Molecular and Developmental Evolution 2024年9月12日  査読有り
    ABSTRACT False clownfish (Amphiprion ocellaris) employ a hatching strategy regulated by environmental cues, wherein parents provide water flow to encourage embryos to hatch after sunset on the hatching day. Despite previous studies demonstrating the necessity of complete darkness and water agitation for hatching, the regulatory mechanisms underlying these environmental cues remain elusive. This study aimed to investigate how darkness and water agitation affect the secretion of hatching enzymes and the hatching movements of embryos in false clownfish. Assessment of chorion digestion and live imaging of Ca2+ in hatching glands using GCaMP6s, a Ca2+ indicator, revealed that darkness stimulation triggers the secretion of hatching enzymes by increasing Ca2+ levels in hatching gland cells. On the other hand, water agitation primarily stimulated hatching movements in embryos, which led to the rupture of their egg envelopes. These results suggest that changes in light environments following sunset induce embryos to secrete hatching enzymes and that water agitation provided by parents stimulates hatching movements. These responses to environmental cues, light and water agitation, contribute to the rapid and synchronous hatching in false clownfish.
  • Shunsuke Nishio, Chihiro Emori, Benjamin Wiseman, Dirk Fahrenkamp, Elisa Dioguardi, Sara Zamora-Caballero, Marcel Bokhove, Ling Han, Alena Stsiapanava, Blanca Algarra, Yonggang Lu, Mayo Kodani, Rachel E. Bainbridge, Kayla M. Komondor, Anne E. Carlson, Michael Landreh, Daniele de Sanctis, Shigeki Yasumasu, Masahito Ikawa, Luca Jovine
    Cell 187(6) 1440-1459.e24 2024年3月  査読有り
  • Mari Kawaguchi, Wen-Shan Chang, Hazuki Tsuchiya, Nana Kinoshita, Akira Miyaji, Ryouka Kawahara-Miki, Kenji Tomita, Atsushi Sogabe, Makiko Yorifuji, Tomohiro Kono, Toyoji Kaneko, Shigeki Yasumasu
    Cell and Tissue Research 2023年5月25日  査読有り
  • Yuko Nakano, Tatsuki Nagasawa, Yohei Okazawa, Naoya Mashiko, Shigeki Yasumasu, Mari Kawaguchi
    Journal of Experimental Zoology Part B: Molecular and Developmental Evolution 2023年4月24日  査読有り

MISC

 27

書籍等出版物

 8

共同研究・競争的資金等の研究課題

 18