JOURNAL OF INORGANIC BIOCHEMISTRY 176 140-143 2017年11月
We have determined a crystal structure of an RNA duplex containing a novel metal-binding motif. The motif is composed of two sheared GOA base pairs, two unpaired A residues and four phosphate groups in close proximity. Four A residues make an A-A-...
D-3-Hydroxybutyrate dehydrogenase catalyzes the reversible conversion of acetoacetate and D-3-hydroxybutyrate. These ketone bodies are both energy-storage forms of acetyl-CoA. In order to clarify the structural mechanisms of the catalytic reaction...
Juan Pablo Maianti   Hiroki Kanazawa   Paola Dozzo   Rowena D. Matias   Lee Ann Feeney   Eliana S. Armstrong   Darin J. Hildebrandt   Timothy R. Kane   Micah J. Gliedt   Adam A. Goldblum   Martin S. Linsell   James B. Aggen   Jiro Kondo   Stephen Hanessian   
ACS CHEMICAL BIOLOGY 9(9) 2067-2073 2014年9月 [査読有り]
Aminoglycoside antibiotics are pseudosaccharides decorated with ammonium groups that are critical for their potent broad-spectrum antibacterial activity. Despite over three decades of speculation whether or not modulation of pK(a) is a viable stra...
S. Hanessian   O. M. Saavedra   M. A. Vilchis-Reyes   J. P. Maianti   H. Kanazawa   P. Dozzo   R. D. Matias   A. Serio   J. Kondo   
CHEMICAL SCIENCE 5(12) 4621-4632 2014年
This study reports the synthesis, antibacterial evaluation and nature of fluorine-rRNA contacts revealed by an X-ray co-crystal structure of a series of 4'-deoxy-4'-fluoro B-neomycin analogs. 4'-Deoxyfluorination improves the inhibition profile to...