理工学部

Kanzawa Nobuyuki

  (神澤 信行)

Profile Information

Affiliation
Professor, Faculty of Science and Technology, Department of Materials and Life Sciences, Sophia University
Degree
PhD(Mar, 1996, Chiba University)

Researcher number
40286761
J-GLOBAL ID
200901047892599780
researchmap Member ID
1000212244

(Subject of research)
Investigation of the Mechanism of Plant Nastic Movement.
Biochemical evaluation of a novel apatite fiber scaffold.


Major Research Interests

 7

Papers

 111
  • Satoshi Kawamura, Kozue Furuya, Nene Sasaki, Yuko Takeoka, Mamoru Aizawa, Nobuyuki Kanzawa
    Journal of biomedical materials research. Part B, Applied biomaterials, 112(6) e35433, Jun, 2024  Peer-reviewedCorresponding author
    Ex vivo tissue engineering is an effective therapeutic approach for the treatment of severe cartilage diseases that require tissue replenishment or replacement. This strategy demands scaffolds that are durable enough for long-term cell culture to form artificial tissue. Additionally, such scaffolds must be biocompatible to prevent the transplanted matrix from taking a toll on the patient's body. From the viewpoint of structure and bio-absorbability, a β-tricalcium phosphate (β-TCP) fiber scaffold (βTFS) is expected to serve as a good scaffold for tissue engineering. However, the fragility and high solubility of β-TCP fibers make this matrix unsuitable for long-term cell culture. To solve this problem, we developed an alginate-coated β-TCP fiber scaffold (βTFS-Alg). To assess cell proliferation and differentiation in the presence of βTFS-Alg, we characterized ATDC5 cells, a chondrocyte-like cell line, when grown in this matrix. We found that alginate coated the surface of βTFS fiber and suppressed the elution of Ca2+ from β-TCP fibers. Due to the decreased solubility of βTFS-Alg compared with β-TCP, the former provided an improved scaffold for long-term cell culture. Additionally, we observed superior cell proliferation and upregulation of chondrogenesis marker genes in ATDC5 cells cultured in βTFS-Alg. These results suggest that βTFS-Alg is suitable for application in tissue culture.
  • Erika Onuma, Hayato Ito, Maki Sasaki, Nobuyuki Kanzawa, Keiji Kito, Mamoru Aizawa
    Materialia, 32 101926-101926, Dec, 2023  Peer-reviewed
  • Erika Onuma, Takayuki Honda, Hideyuki Yoshimura, Tappei Nishihara, Atsushi Ogura, Nobuyuki Kanzawa, Mamoru Aizawa
    Crystals, 13(9) 1318-1318, Aug 29, 2023  Peer-reviewed
    Protein adsorption is essential for determining material biocompatibility and promoting adherent cell growth. In this study, we focused on the a-plane structure of hydroxyapatite (HAp). This a-plane structure closely resembles the crystal plane where apatite is exposed in long bones. We conducted protein adsorption experiments using HAp ceramics with a preferred orientation to a-planes (aHAp), employing bovine serum albumin (BSA), lysozyme, and fetal bovine serum (FBS) as protein models to mimic the in vivo environment. Higher zeta potential and contact angle values were found in aHAp than in HAp ceramics fabricated from commercial HAp powder (iHAp). Bradford-quantified protein adsorption revealed BSA adsorption of 212 ng·mm−2 in aHAp and 28.4 ng mm−2 in iHAp. Furthermore, the Bradford-quantified protein adsorption values for FBS were 2.07 μg mm−2 in aHAp and 1.28 µg mm−2 in iHAp. Two-dimensional electrophoresis (2D-PAGE) showed a higher number of protein-derived major spots in aHAp (37 spots) than in iHAp (12 spots). Mass spectrometry analysis of the resulting 2D-PAGE gels revealed proteins adsorbed on aHAp, including secreted frizzled-related protein 3 and vitamin K epoxide reductase complex 1, which are involved in cellular bone differentiation. Overall, these proteins are expected to promote bone differentiation, representing a characteristic property of aHAp.
  • Ayame Mikagi, Yotaro Takahashi, Nobuyuki Kanzawa, Yota Suzuki, Yuji Tsuchido, Takeshi Hashimoto, Takashi Hayashita
    Molecules, 28(4) 1704, Feb, 2023  Peer-reviewed
  • Ayame Mikagi, Koichi Manita, Yuji Tsuchido, Nobuyuki Kanzawa, Takeshi Hashimoto, Takashi Hayashita
    ACS Applied Bio Materials, Nov 1, 2022  Peer-reviewed

Misc.

 23

Books and Other Publications

 5

Presentations

 55

Professional Memberships

 7

Research Projects

 16

Industrial Property Rights

 1

Social Activities

 6