Faculty of Science and Technology

臼杵 豊展

ウスキ トヨノブ  (Toyonobu Usuki)

基本情報

所属
上智大学 理工学部物質生命理工学科 教授
学位
学士(理学)(東北大学)
修士(理学)(東北大学)
博士(理学)(東北大学)

連絡先
t-usukisophia.ac.jp
研究者番号
50514535
J-GLOBAL ID
200901076489387829
researchmap会員ID
5000046104

学歴

 3

論文

 116
  • Khin Khin Gyi, Songyot Anuchapreeda, Nutjeera Intasai, Montree Tungjai, Siriporn Okonogi, Arihiro Iwasaki, Toyonobu Usuki, Singkome Tima
    BMC complementary medicine and therapies 25(1) 172-172 2025年5月13日  
    BACKGROUND: Gynostemma pentaphyllum (Thunb.), a traditional adaptogenic herb, is known for its bioactive components with potential anti-cancer properties. Acute myeloid leukemia (AML) progression is significantly influenced by Feline McDonough Sarcoma (FMS)-like tyrosine kinase 3 (FLT3) signaling, while Wilms' tumor 1 (WT1) serves as a key prognostic marker. This study investigates the anti-leukemia activities of active G. pentaphyllum leaf extracts and their components, focusing on the inhibition of FLT3 and WT1 activity. METHODS: G. pentaphyllum extracts were prepared through maceration, yielding three crude fractional extracts. The cytotoxicity of the extracts was screened against various leukemia cell lines using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. The most cytotoxic extract was further fractionated and purified via column chromatography. The anti-proliferative and apoptotic induction activities of the active extract and its fraction were evaluated through cell cycle and apoptosis analyses using flow cytometry. Changes in mitochondrial membrane potential (ΔΨm) were assessed by spectrofluorometry. To confirm anti-leukemia activity, the expression levels of FLT3, WT1 and apoptotic-related protein were analyzed using Western blotting. The major active compounds within the active fractions were identified and characterized using Electrospray Ionization Mass Spectrometry (ESI-MS) and Nuclear Magnetic Resonance (NMR) spectroscopy. RESULTS: The ethyl acetate fractional extract (F-EtOAc) demonstrated the highest cytotoxicity, particularly against FLT3-overexpressing EoL-1 (IC50 = 40.82 ± 0.8 µg/mL) and MV4-11 (IC50 = 35.54 ± 4.1 µg/mL) AML cell lines. Fraction F10 was identified as the most active fraction, significantly inhibited FLT3 and WT1 protein expression and induced G0/G1 cell cycle arrest in a dose-dependent manner. Additionally, F10 induced dose-dependent apoptosis through disruption of ΔΨm, p53 up-regulation and caspase-3 activation. Further purification of F10 identified dehydrovomifoliol as its major bioactive compound. CONCLUSION: These findings suggest that the ethyl acetate extract of G. pentaphyllum contains bioactive compounds with anti-leukemia potential, warranting further investigation to evaluate its efficacy against AML. CLINICAL TRIAL NUMBER: Not applicable.
  • Hiroaki Ishii, Ryosuke Shigeta, Kaho Oishi, Toyonobu Usuki
    Tetrahedron Letters 158 155508-155508 2025年3月  査読有り責任著者
  • Futa Koyama, Justin Lamb, Moena Hirao, Noriyuki Suzuki, Masahiro Yoshizawa-Fujita, Toyonobu Usuki, Yumiko Suzuki
    Advanced Synthesis & Catalysis 2024年10月4日  
    With a number of biologically active members, 2‐aroylchromones are valuable synthetic targets. A direct route towards 2‐aroylchromones from 2‐(methylsulfonyl)chromones and aldehydes via NHC‐catalyzed C‐C bond formation was developed. Yields of the synthesized 2‐aroylchromones were up to 85%. Chromones with angioprotective or antibacterial properties were easily synthesized using the method developed. Additionally, the synthetic utility of the afforded chromones was demonstrated by using them to synthesize the anticancer compound wrightiadione and analogues of it.
  • Christian Nanga Chick, Francois Eya'ane Meva, Phillippe Belle Ebanda Kedi, Toyonobu Usuki
    2024年7月17日  査読有り責任著者
  • Eri Tanaka, Yukie Yokota, Masamitsu Urakawa, Toyonobu Usuki
    European Food Research and Technology 2024年4月21日  査読有り責任著者

MISC

 3

書籍等出版物

 2

講演・口頭発表等

 283

共同研究・競争的資金等の研究課題

 25

その他

 7