理工学部

Kawaguchi Mari

  (川口 眞理)

Profile Information

Affiliation
Associate Professor, Faculty of Science and Technology, Department of Materials and Life Sciences, Sophia University
Degree
修士(理学)(東京都立大学)
博士(理学)(上智大学)

Researcher number
00612095
J-GLOBAL ID
201301084945465719
researchmap Member ID
7000004358

External link

2008年~2011年 東京大学大気海洋研究所
   「孵化酵素と卵膜の分子共進化」

2011年~現在 上智大学理工学部物質生命理工学科
   「魚類の繁殖戦略の進化」

(研究テーマ)
魚類の繁殖戦略の進化


Committee Memberships

 9

Papers

 49
  • Shigeki Yasumasu, Miyuki Horie, Mayuko Horie, Kodai Sakuma, Chihiro Sato, Hikari Sato, Taiki Nakajima, Tatsuki Nagasawa, Mari Kawaguchi, Ichiro Iuchi
    The Journal of Biochemistry, Sep 16, 2024  Peer-reviewed
    Abstract During the fertilization of fish eggs, the hardening of the egg envelope is mediated by transglutaminase (hTGase). After fertilization, TGase undergoes processing. We isolated hTGase from extracts of unfertilized and water-activated rainbow trout eggs. Rainbow trout hTGase (Rt-hTGase) appeared as an 80 kDa protein, and its processed form was 55 kDa. Their N-terminal amino acid sequences were nearly identical, suggesting processing in the C-terminal region. The specific activities were not significantly different, indicating that C-terminal processing does not activate the enzyme itself. We cloned the cDNA by reverse transcription polymerase chain reaction (RT-PCR) using degenerate primers followed by RACE-PCR. The deduced amino acid sequence of the cDNA was similar to that of factor XIII subunit A (FXIIIA). Molecular phylogenetic and gene syntenic analyses clearly showed that hTGase was produced by duplication of FXIIIA during the evolution to Teleostei. The 55 kDa processed form of Rt-hTGase is predominantly composed of an enzyme domain predicted from the amino acid sequence of the cDNA. It is hypothesized that the C-terminal domain of Rt-hTGase binds to egg envelope proteins, and that processing allows the enzyme to move freely within the egg envelope, increasing substrate–enzyme interaction and thereby accelerating hardening.
  • Sakuto Yamanaka, Mari Kawaguchi, Shigeki Yasumasu, Kenji Sato, Masato Kinoshita
    Journal of Experimental Zoology Part B: Molecular and Developmental Evolution, Sep 12, 2024  Peer-reviewed
  • Christian Nanga Chick, Yusuke Sasaki, Mari Kawaguchi, Eri Tanaka, Takako Niikura, Toyonobu Usuki
    Bioorganic & Medicinal Chemistry, 90 117351-117351, Jul, 2023  Peer-reviewedCorresponding author
  • Mari Kawaguchi, Wen-Shan Chang, Hazuki Tsuchiya, Nana Kinoshita, Akira Miyaji, Ryouka Kawahara-Miki, Kenji Tomita, Atsushi Sogabe, Makiko Yorifuji, Tomohiro Kono, Toyoji Kaneko, Shigeki Yasumasu
    Cell and Tissue Research, May 25, 2023  Peer-reviewedLead authorCorresponding author
  • Masahiro Sano, Hikaru Iwashita, Chihiro Suzuki, Mari Kawaguchi, Atsuhiko Chiba
    NeuroReport, 34(9) 457-462, May 10, 2023  Peer-reviewed

Misc.

 12

Books and Other Publications

 5

Presentations

 143

Research Projects

 21

Social Activities

 5

Media Coverage

 16